Carbonyl sulfide: an inhibitor of inorganic carbon transport in cyanobacteria.
نویسندگان
چکیده
Cells of a high CO(2)-requiring mutant (E(1)) and wild type of Synechococcus PCC7942 were incubated with COS in the light, then suspended in COS-free medium and their CO(2) exchange was measured using an open gas-analysis system under the conditions where photosynthetic CO(2) fixation is inhibited. When the suspension of cells untreated with COS was illuminated, the rate of CO(2) uptake was high and addition of carbonic anhydrase during illumination released a large amount of CO(2) from the medium into the gas phase. The COS treatment in the light markedly reduced the rate of CO(2) uptake by the cells and the amount of CO(2) released by carbonic anhydrase. Incubation of cells with COS in the dark had no effect on the CO(2)-exchange profile. The COS concentration required for 50% inhibition of CO(2) uptake was about 25 micromolar when the concentration of inorganic carbon (C(i)) in the medium was 60 micromolar; higher C(i) concentrations reduced the inhibitory effect of COS. Measurement of C(i) uptake in E(1) cells by a silicone oil centrifugation method also indicated marked reduction of the activities of (14)CO(2) and H(14)CO(3) (-) uptake in the cells treated with COS in the light. The results demonstrated that COS is a potent inhibitor of C(i) transport.
منابع مشابه
A coupled model of the global cycles of carbonyl sulfide and CO2: A possible new window on the carbon cycle
[1] Carbonyl sulfide (COS) is an atmospheric trace gas that participates in some key reactions of the carbon cycle and thus holds great promise for studies of carbon cycle processes. Global monitoring networks and atmospheric sampling programs provide concurrent data on COS and CO2 concentrations in the free troposphere and atmospheric boundary layer over vegetated areas. Here we present a mode...
متن کاملA Theoretical Study of H2S and CO2 Interaction with the Single-Walled Nitrogen Doped Carbon Nanotubes
The physical adsorption of hydrogen sulfide and carbon dioxide gases on the zigzag (5,0) carbon nanotubes doped with nitrogen was investigated through the application of B3LYP/6-31G* at the level of theory on Gaussian 03 software. A variety of stable and high abundance structures of nitrogen doped carbon nanotubes were considered in order to study the interaction between the mentioned gases in ...
متن کاملA Theoretical Study of H2S and CO2 Interaction with the Single-Walled Nitrogen Doped Carbon Nanotubes
The physical adsorption of hydrogen sulfide and carbon dioxide gases on the zigzag (5,0) carbon nanotubes doped with nitrogen was investigated through the application of B3LYP/6-31G* at the level of theory on Gaussian 03 software. A variety of stable and high abundance structures of nitrogen doped carbon nanotubes were considered in order to study the interaction between the mentioned gases in ...
متن کاملCharacterization of sulfate transport in cultured tobacco cells.
Sulfate transport by tobacco cells (Nicotiana tabacum L. var. Xanthi) cultured in liquid medium was investigated.Transport was linear with time, had a sharp pH optimum between 6.5 and 7.5, and obeyed Michaelis-Menten kinetics. The Km varied within the range 2 x 10(-5)m and 4 x 10(-5)m and the maximum velocity was in the range 100 to 400 nanomoles per gram fresh weight.hour.Transport was inhibit...
متن کاملThe flux of carbonyl sulfide and carbon disulfide
The flux of carbonyl sulfide and carbon disulfide between the atmosphere and a spruce forest X. Xu, H. G. Bingemer, and U. Schmidt Institute for Meteorology and Geophysics, University of Frankfurt, P.O. Box 111932, 60054 Frankfurt am Main, Germany now at Max Planck Institute for Chemistry, Mainz, Germany Received: 9 January 2002 – Accepted: 19 February 2002 – Published: 21 February 2002 Corresp...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Plant physiology
دوره 88 3 شماره
صفحات -
تاریخ انتشار 1988